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Cambridge Immunology Network

 

Research

Supervisor: Dr John James

I am currently working to better understand how the pre-T cell receptor (TCR) differs at a molecular and mechanistic level during early thymocyte development and β-selection checkpoint.  Utilising a reconstituted pre-TCR system to manipulate signalling in both HEK293T cells and in vitro differentiated thymocytes with lentiviral transduction followed by flow cytometry and microscopy I determine the cellular localisation and developmental effects of TCR complexes.

Supervisor: Dr Martin Turner

I am interested in the role of post-transcriptional mechanisms that govern and control early T-cell differentiation.  The Turner lab has previously created a conditional double knock-out of two RNA binding proteins; Tis11b (Zfp36l1) and Tis11d (Zfp36l2).  This conditional dKO develops a severe T-acute lymphoblastic like leukaemia (T-ALL) like disease but has perturbed thymic development prior to disease onset.  My PhD project is focussing on the mechanisms behind the control of early thymocyte differentiation and development by these RNA binding proteins.

Publications

Key publications: 
 Lewis  Bell
Not available for consultancy

Affiliations

Classifications: 
Departments and institutes: 
Person keywords: 
T lymphocyte
thymocytes
mRNA decay
microscopy
cell signalling
Gamma Delta T cells
ubiquitin E3 ligase
cell development
ZAP-70
mouse immunology
flow cytometry
cellular immunology
cell biology
immune development
T cell receptor (TCR)
TCR triggering
AREBP
live cell imaging
lymphocyte activation
development
molecular biology
immune regulation
T cell differentiation
activation
T cells
gamma delta T cells
cell fate decision
lymphocytes,
immune cell differentiation
3′ UTR
immune response
ligands
lymphoma
immune signalling
T-cell receptor complex
signal transduction
cell culture
cell differentiation
fluorescence microscopy
stem cells
LAT
FACS
signalling
mRNA targets
optogenetics