Cambridge Immunology Network
Automatic single cell image analysis to measure the translocation of the pro-inflammatory transcription factor Nuclear Factor kappa B into the nucleus and the accompanying expression of the cytokine tumor necrosis factor alpha over time after stimulation of cells with lipopolysaccharide. Macrophages are transduced to express green fluorescent protein-Rel A (a constituent of Nuclear Factor kappa B) and a tumor necrosis factor alpha promoter linked to mCherry (red)
Cambridge Immunology Network Coordinator
University of Cambridge Department of Medicine,
Jeffrey Cheah Biomedical Centre,
Cambridge Biomedical Campus, Puddicombe Way,
Cambridge, CB2 0AW
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