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Cambridge Immunology Network

 
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Automatic single cell image analysis to measure the translocation of the pro-inflammatory transcription factor Nuclear Factor kappa B into the nucleus and the accompanying expression of the cytokine tumor necrosis factor alpha over time after stimulation of cells with lipopolysaccharide. Macrophages are transduced to express green fluorescent protein-Rel A (a constituent of Nuclear Factor kappa B) and a tumor necrosis factor alpha promoter linked to mCherry (red)