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Dr Anne Corcoran

Dr Anne Corcoran

Anne Corcoran is accepting applications for PhD students.

Anne Corcoran is available for consultancy.


Research Interests

My group studies the chromatin remodelling mechanisms that underlie V(D)J recombination and allelic exclusion of antigen receptor loci, in particular the immunoglobulin heavy and light chain loci. We have shown that large-scale non-coding RNA transcription (antisense and intergenic) precedes both D to J and V to DJ recombination at regions poised for recombination, and have proposed that this process opens up the chromatin to facilitiate stage-specific V(D)J recombination. We have developed RNA-FISH techniques to visualise primary transcription on individual Ig alleles in single cells. We are also interested in the nuclear organisation of V(D)J recombination, and in understanding events that predispose to generation of chromosomal translocations of the Ig loci and B cell lymphoma.

Keywords

cytokine/interleukin/chemokine receptors ; B cells ; chromosome conformation capture (3C) ; immunosenescence ; microscopy ; confocal microscopy ; differentiation ; T cell receptor (TCR) ; cytokines ; aging ; B cell receptor (BCR) ; fluorescence microscopy ; chromatin ; non-coding RNA ; immunoglobulins ; human studies ; affinity maturation ; antibody responses ; animal models ; chromatin immunoprecipitation ; allelic exclusion ; V(D)J recombination ; isotype switching ; cell culture ; cell development ; RNA and DNA FISH ; somatic hypermutation ; FACS

Key Publications

Bolland DJ, King MR, Reik W, Corcoran AE, Krueger C. Robust 3D DNA FISH using directly labeled probes. J Vis Exp. 2013 Aug 15;(78).

Matheson LS, Corcoran AE. Local and global epigenetic regulation of V(D)J recombination. Curr Top Microbiol Immunol. 2012;356:65-89

Corcoran AE. The epigenetic role of non-coding RNA transcription and nuclear organization in immunoglobulin repertoire generation. Semin Immunol. 2010 Dec;22(6):353-61.

Ramadani F, Bolland DJ, Garcon F, Emery JL, Vanhaesebroeck B, Corcoran AE, Okkenhaug K.  The PI3K isoforms p110alpha and p110delta are essential for pre-B cell receptor signaling and B cell development. Sci Signal. 2010 Aug 10;3(134

Featherstone K, Wood AL, Bowen AJ, Corcoran AE.The mouse immunoglobulin heavy chain V-D intergenic sequence contains insulators that may regulate ordered V(D)J recombination. J Biol Chem. 2010 Mar 26;285(13):9327-38.

Bolland DJ, Wood AL, Corcoran AE.Large-scale chromatin remodeling at the immunoglobulin heavy chain locus: a paradigm for multigene regulation. Adv Exp Med Biol. 2009;650:59-72.

Bowen AJ, Corcoran AE. How chromatin remodelling allows shuffling of immunoglobulin heavy chain genes. Mol Biosyst. 2008 Aug;4(8):790-8.