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Devina Divekar

Research Interests

Research assistant to Dr Michelle Linterman

At present I am involved in the projects looking at the germinal cell response which is a key factor for production of high affinity antibodies and generation of immunological memory. I get involved with histology and immunohistochemistry for generating quality data representing and validating the results obtained by FACS.

spleen col

Spleen section from immunised mice at day7 stained with  an anti-IgD(red) , anti Bcl6 (green), an anti CD4 (blue) and Foxp3 (white).The B cell follicle is stained with an anti-IgD. CD4+ Foxp3+  regulatory T cells (T-regs) can be seen at the T-B  border.

Keywords

FOXP3 ; flow cytometry ; molecular biology ; germinal center ; T cells ; immunohistochemistry ; regulatory T cells ; protein analysis ; histology ; confocal microscopy

Key Publications

Linterman MA, Pierson W, Lee SK, Kallies A, Kawamoto S, Rayner TF, Srivastava M, Divekar DP, Beaton L, Hogan JJ, Fagarasan S, Liston A, Smith KG, Vinuesa CG. Foxp3+ follicular regulatory T cells control the germinal center response. Nat Med. 2011 Jul 24;17(8):975-82. doi: 10.1038/nm.2425.

Tile scan of spleen section from immunised mouse at day7 stained with  an anti-IgD(red) , anti-Bcl6 (green) ,an anti-CD4 (blue) and Foxp3 (white). Picture represents the germinal centre formation at various locations which is identified by positive IgD staining around the B cell follicle and surrounded by CD4 + Foxp3+ T cell zone.

Spleen section from immunised mice at day7 stained with  an anti-IgD(red) , anti Bcl6 (green) and an anti CD4 (blue) .The B cell follicle is stained with an anti-IgD whereas the germinal centre B cells are positive for BCL6. The differentiating T follicular helper (Tfh) cells can be identified by CD4+ BCL6+ staining.