Research assistant to Dr Michelle Linterman
At present I am involved in the projects looking at the germinal cell response which is a key factor for production of high affinity antibodies and generation of immunological memory. I get involved with histology and immunohistochemistry for generating quality data representing and validating the results obtained by FACS.
Spleen section from immunised mice at day7 stained with an anti-IgD(red) , anti Bcl6 (green), an anti CD4 (blue) and Foxp3 (white).The B cell follicle is stained with an anti-IgD. CD4+ Foxp3+ regulatory T cells (T-regs) can be seen at the T-B border.
FOXP3 ; flow cytometry ; molecular biology ; germinal center ; T cells ; immunohistochemistry ; regulatory T cells ; protein analysis ; histology ; confocal microscopy
Linterman MA, Pierson W, Lee SK, Kallies A, Kawamoto S, Rayner TF, Srivastava M, Divekar DP, Beaton L, Hogan JJ, Fagarasan S, Liston A, Smith KG, Vinuesa CG. Foxp3+ follicular regulatory T cells control the germinal center response. Nat Med. 2011 Jul 24;17(8):975-82. doi: 10.1038/nm.2425.