Supervisor: Professor David Klenerman
During my PhD I have built and characterised a PALM/STORM super-resolution microscope with Dr Steven F Lee to further investigate the formation of the contact between a T cell and either a glass IgG-coated coverslip, a lipid bilayer or an antigen-presenting cell. The super-resolution instrument unravels the very early events leading to TCR triggering giving snapshots of contact zones with a 10 nm resolution. This helps us study undescribed ring structures characteristic of the kinetic-segregation hypothesis, in collaboration with Professor Simon Davis in Oxford. I am finally also developing a method to precisely count the number of proteins in a contact zone to study the aggregation hypothesis.
super-resolution ; T cells ; MHC ; single molecule imaging ; TCR triggering ; kinetic segregation ; antigen presentation ; T cell receptor (TCR)
Etheridge TJ, Boulineau R, Herbert A, Watson AT, Daigaku Y, Tucker J, George S, Jönsson P, Palayret M, Lando D, Laue E, Osborne MA, Klenerman D, Lee SF, Carr AM. Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy. Nucl. Acids Res. 2014;gku726-
Review: Palayret M, Horrocks MH, Klenerman D, Lee SF. The changing point-spread-function: single-molecule based super-resolution imaging. Histochem. Cell Biol. 2014 Jun;141:6(577-85)
Bell L, Seshia A, Lando D, Laue E, Palayret M, Lee SF, Klenerman D. A microfluidic device for the hydrodynamic immobilisation of living yeast cells for super-resolution imaging. Sensor Actuat. B-Chem. 2014;192(36-41)