My research focus is to gain a better understanding of the mechanisms controlling inflammatory processes with a view to help develop novel strategies for inflammatory diseases. Although eosinophilic granulocytes are key effector cells in host defence against invading parasites, over-recruitment, uncontrolled activation and defective removal of these cells plays a major role in the initiation and propagation of inflammatory conditions including asthma and allergic rhinitis. I am currently investigating eosinophil trafficking in humans and specifically examining the size and distribution of marginating eosinophil pools, the half-life of the eosinophil in blood and its ultimate fate.
enzyme assays ; eosinophils ; radioactvity ; microscopy ; apoptosis ; human studies ; cell culture ; animal models ; inflammation ; FACS ; neutrophils
Farahi N, Loutsios C, Peters AM, Condliffe AM, Chilvers ER.Use of Technetium-99m-labeled Eosinophils to Detect Active Eosinophilic Inflammation in Humans. Am J Respir Crit Care Med. 2013 Oct 1;188(7):880-882
Farahi N, Singh NR, Heard S, Loutsios C, Summers C, Solanki CK, Solanki K, Balan KK, Ruparelia P, Peters AM, Condliffe AM, Chilvers ER. Use of 111-Indium-labelled autologous eosinophils to establish the in vivo kinetics of human eosinophils in healthy subjects. Blood. 2012 Sep 19.
Farahi N, Uller L, Juss JK, Langton AJ, Cowburn AS, Gibson A, Foster MR, Farrow SN, Marco-Casanova P, Sobolewski A, Condliffe AM, Chilvers ER. Effects of the cyclin-dependent kinase inhibitor R-roscovitine on eosinophil survival and clearance. Clin Exp Allergy. 2011 May;41(5):673-87
Farahi, N, Cowburn AS, Upton PD, Deighton J, Sobolewski A, Gherardi E, Morrell NW and Chilvers ER . Eotaxin-1/CC Chemokine Ligand 11: a novel eosinophil survival factor secreted by human pulmonary artery endothelial cells. J, Immunol. 2007.179:1264–1273.
Walmsley, SR, C Print, N Farahi, C Peyssonnaux, RS Johnson, T Cramer, A Sobolewski, AM Condliffe, AS Cowburn, N Johnson and ER Chilvers. Hypoxia-induced neutrophil survival is mediated by HIF-1alpha-dependent NF-kappaB activity. J, Exp. Med. 2005 201:105–115.
Farahi, N, AS Cowburn, AG Rossi and ER Chilvers. Eating their way out of trouble: selective uptake of apoptotic eosinophils by bronchial epithelial cells. Clin. Exp. Allergy 2004. 34:1503–1506.