Sarah Teichmann is accepting applications for PhD students.
Sarah Teichmann is available for consultancy.
The central question we seek to answer is: how are changes in cell state regulated at the transcriptomic and epigenetic level? T helper cells are ideal to address this question, because the Th system consists of a naïve precursor cell type, and four main differentiated cell types that have some capacity to interconvert, known as plasticity. We use an integrated computational and experimental approach, including genome-wide approaches such as RNA-sequencing and ChIP-sequencing, and single cell techniques such as single molecule RNA-FISH.
By understanding the intricate control of changes in gene expression that these cells undergo as they differentiate or reprogramme, we hope to also gain insight into the biology of infection, allergy and autoimmune diseases.
genomics ; transcriptional regulatory networks ; T helper cells ; cell culture ; next generation sequencing ; RNA and DNA FISH ; CD4+ ; bioinformatics ; FACS ; confocal microscopy ; epigenomics ; helper T cells
Mahata B, Zhang X, Kolodziejczyk AA, Proserpio V, Haim-Vilmovsky L, Taylor AE, Hebenstreit D, Dingler FA, Moignard V, Göttgens B, Arlt W, McKenzie AN, Teichmann SA. Single-cell RNA sequencing reveals T helper cells synthesizing steroids de novo to contribute to immune homeostasis. Cell Rep. 2014 May 22;7(4):1130-42
Feig C, Jones JO, Kraman M, Wells RJ, Deonarine A, Chan DS, Connell CM, Roberts EW, Zhao Q, Caballero OL, Teichmann SA, Janowitz T, Jodrell DI,Tuveson DA, Fearon DT. Targeting CXCL12 from FAP-expressing carcinoma-associated fibroblasts synergizes with anti-PD-L1 immunotherapy in pancreatic cancer. Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20212-7.
Roberts EW, Deonarine A, Jones JO, Denton AE, Feig C, Lyons SK, Espeli M, Kraman M, McKenna B, Wells RJ, Zhao Q, Caballero OL, Larder R, Coll AP, O'Rahilly S, Brindle KM, Teichmann SA, Tuveson DA, Fearon DT. Depletion of stromal cells expressing fibroblast activation protein-α from skeletal muscle and bone marrow results in cachexia and anemia. J Exp Med. 2013 May 27.
Hebenstreit D, Deonarine A, Babu MM, Teichmann SA. Duel of the fates: the role of transcriptional circuits and noise in CD4+ cells. Curr Opin Cell Biol. 2012 Jun;24(3):350-8.
Hebenstreit D, Fang M, Gu M, Charoensawan V, van Oudenaarden A, Teichmann SA. RNA sequencing reveals two major classes of gene expression levels in metazoan cells.Mol Syst Biol. 2011 Jun 7;7:497.
Hebenstreit D, Gu M, Haider S, Turner DJ, Liò P, Teichmann SA EpiChIP: gene-by-gene quantification of epigenetic modification levels. Nucleic Acids Res 2010.
Vaquerizas JM, Kummerfeld SK, Teichmann SA, Luscombe NM, A census of human transcription factors: function, expression and evolution Nature Reviews Genetics 2009 10(4):252–63