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Cambridge Immunology Network

 

Research

Cytotoxic T-lymphocytes (CTL) kill tumourogenic and virally infected cells by the polarised release of apoptotic-inducing lytic proteins stored within the ‘secretory’ lysosomes of the CTL. Secretion occurs in response to TcR activation on binding to a target cell, and involves morphological and molecular changes within the CTL including movement of the centrosome to the cell surface. These changes result in directed delivery of secretory lysosomes to specific secretory sites on the CTL membrane.

My research as a cell biologist in the laboratory of Professor Gillian Griffiths, takes morphological approaches, including electron and immunofluorescence microscopy, to investigate the cellular events and mechanisms involved in this precisely targeted secretion event.

Publications

Key publications: 

Stinchcombe JC, Salio M, Cerundolo V, Pende D, Arico M, Griffiths GM. Centriole polarisation to the immunological synapse directs secretion from cytolytic cells of both the innate and adaptive immune systems. 2011.  BMC Biol. 9:45

Tsun A, Qureshi I, Stinchcombe JC, Jenkins MR, de la Roche M, Kleczkowska J, Zamoyska R, Griffiths GM. Centrosome docking at the immunological synapse is controlled by Lck signalling. 2011. J Cell Biol. 192:663-74

Griffiths GM, Tsun A, Stinchcombe JC. The immunological synapse: a focal point for endocytosis and exocytosis. 2010.   J Cell Biol 189:399-406. Review.

Jenkins MR, Tsun A, Stinchcombe JC, Griffiths GM.The strength of T cell receptor signal controls the polarization of cytotoxic machinery to the immunological synapse. 2009.  Immunity. 31:621-31

Stinchcombe JC, Griffiths GM. Secretory mechanisms in cell-mediated cytotoxicity. 2007. Annu Rev Cell Dev Biol. 23:495-517. Review.

Stinchcombe JC, Majorovits E, Bossi G, Fuller S, Griffiths GM. Centrosome polarization delivers secretory granules to the immunological synapse. 2006. Nature. 443:462-5

Not available for consultancy

Affiliations

Classifications: 
Departments and institutes: 
Person keywords: 
cell biology
microscopy
centrosome polarisation
CTL
secretory lysosome secretion
immunological synapse