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Dr Stephen Graham

Dr Stephen Graham

Sir Henry Dale Fellow

University Lecturer

Stephen Graham is accepting applications for PhD students.

Stephen Graham is available for consultancy.


Research Interests

The compartmentalisation characteristic of eukaryotic cells presents a logistical dilemma: How do cells ensure components are efficiently delivered to only the correct intracellular compartment? My lab seeks to understand how multiprotein complexes regulate fusion of compartments in the eukaryotic endocytic pathway.

Viruses that seek to exploit the rich environment inside host cells face a similar dilemma: How can they subvert host membrane trafficking to ensure progeny virions efficiently exit infected cells? I am investigating how viruses that acquire double membrane envelopes within cells are trafficked to and fuse with the plasma membrane.

Lastly, in order to establish a fruitful infection viruses must evade the immune surveillance systems of the host. I am interested in how large double-stranded DNA viruses have hijacked proteins of the mammalian innate immune system and ‘reprogrammed’ these proteins to suit the needs of the virus.

Keywords

virion assembly ; immune evasion ; molecular biology ; host-pathogen interaction ; virology ; pathogen evolution ; structural biology

Topics

  • poxvirus
  • herpesviruses

Key Publications

  1. M.R. Hunter, G.G. Hesketh, T.H. Benedyk, A.C. Gingras, S.C. Graham (2018) Proteomic and biochemical comparison of the cellular interaction partners of human VPS33A and VPS33B. Journal of Molecular Biology, 430: 2153–2163
    doi: 10.1016/j.jmb.2018.05.019
  2. A. Albecka, D.J. Owen, L. Ivanova, J. Brun, R. Liman, L. Davies, M.F. Ahmed, S. Colaco, M. Hollinshead, S.C. Graham^ and C.M. Crump^ (2017) Dual function of the pUL7-pUL51 tegument protein complex in HSV-1 infection. Journal of Virology, 81: e02196-16
    doi: 10.1128/JVI.02196-16
  3. J. Muenzner, L.M. Traub, B.T. Kelly^, S.C. Graham^ (2017) Cellular and viral peptides bind multiple sites on the N-terminal domain of clathrin. Traffic, 18: 44–57
    doi: 10.1111/tra.12457
  4. S. Neidel, C. Maluquer de Motes, D.S. Mansur, P. Strnadova, G.L. Smith, S.C. Graham (2015) Vaccinia Virus Protein A49 is an Unexpected Member of the B-cell Lymphoma (Bcl)-2 Protein Family. Journal of Biological Chemistry, 290: 5991–6002
    doi: 10.1074/jbc.M114.624650
  5. B.T. Kelly, S.C. Graham, N. Liska, P.N. Dannhauser, S. Höning, E.J. Ungewickell, D.J. Owen (2014) AP2 controls clathrin polymerization with a membrane-activated switch. Science, 345: 459–463
    doi: 10.1126/science.1254836

(^Joint corresponding authors)