Research
Stephen is interested in understanding how viruses change the composition and architecture of intracellular membranes, and how they evade immune recognition by the host. His team studies this using a broad range of biochemical, biophysical and cell biology techniques, ranging from cell-based models of infection and proteomics to biophysical analysis and atomic-resolution structures of protein complexes. This research provides fundamental insights into virus biology and virus:host interactions that underpins the development of future anti-viral therapies and virus-based biologics.
Publications
Key publications:
- T.H. Benedyk, V. Connor, E.R. Caroe, M. Shamin, D.I. Svergun, J.E. Deane, C.M. Jeffries, C.M. Crump, S.C. Graham (2022) Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT. Journal of Biological Chemistry, in press [doi: 10.1016/j.jbc.2022.102589]
- K.L. Nahas, V. Connor, K.M. Scherer, C.F. Kaminski, M. Harkiolaki^, C.M. Crump^, S.C. Graham^ (2022) Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of multiple cellular organelles during HSV-1 infection. PLoS Pathogens, 18: e1010629 [doi: 10.1371/journal.ppat.1010629]
- W.N.D. Gao, C. Gao, J.E. Deane, D.C.J. Carpentier, G.L. Smith, S.C. Graham (2022) The crystal structure of vaccinia virus protein E2 and perspectives on the prediction of novel viral protein folds. Journal of General Virology 103: 001716 [doi: 10.1099/jgv.0.001716]
- T.H. Benedyk, J. Muenzner, V. Connor, Y. Han, K. Brown, K.J. Wijesinghe, Y. Zhuang, S. Colaco, G.A. Stoll, O.S. Tutt, S. Svobodova, D.I. Svergun, N.A. Bryant, J.E. Deane, A.E. Firth, C.M. Jeffries, C.M. Crump^, S.C. Graham^ (2021) pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread. PLoS Pathogens, 17: e1009824 [doi: 10.1371/journal.ppat.1009824]
- C. Conceicao*, N. Thakur*, S. Human, J.T. Kelly, L. Logan, D. Bialy, S. Bhat, P. Stevenson-Leggett, A.K. Zagrajek, P. Hollinghurst, M. Varga, C. Tsirigoti, M. Tully, C. Chiu, K. Moffat, A.P. Silesian, J.A. Hammond, H.J. Maier, E. Bickerton, H. Shelton, I. Dietrich, S.C. Graham, D. Bailey (2020) The SARS-CoV-2 Spike protein has a broad tropism for mammalian ACE2 proteins. PLoS Biology 18: e3001016 [doi: 10.1371/journal.pbio.3001016]
- T.K. Soh*, C.T.R. Davies*, J. Muenzner*, L.M. Hunter, H.G. Barrow, V. Connor, C.R. Bouton, C. Smith, E. Emmott, R. Antrobus, S.C. Graham^, M.P. Weekes^, C.M. Crump^ (2020) Temporal Proteomic Analysis of Herpes Simplex Virus 1 Infection Reveals Cell-Surface Remodeling via pUL56-Mediated GOPC Degradation. Cell Reports, 33: 108235 [doi: 10.1016/j.celrep.2020.108235]
- B.G. Butt, D.J. Owen, C.M. Jeffries, L. Ivanova, C.H. Hill, J.W. Houghton, M.F. Ahmed, R. Antrobus, D.I. Svergun, J.J. Welch, C.M. Crump, S.C. Graham (2020) Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex. eLife, 9: e53789 [doi: 10.7554/eLife.53789]
(*Joint first authors, ^Joint corresponding authors)
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