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Cambridge Immunology Network

 

Research

Viruses that seek to exploit the rich environment inside host cells face a logistical dilemma: How can they subvert host membrane trafficking to ensure progeny virions efficiently exit infected cells? I study how viruses modify the composition and architecture of infected cell membranes, with a special emphasis on how viruses that acquire double membrane envelopes within cells are trafficked to and fuse with the plasma membrane.

Additionally, in order to establish a fruitful infection viruses must evade the immune surveillance systems of the host. I am interested in how large double-stranded DNA viruses have hijacked proteins of the mammalian innate immune system and ‘reprogrammed’ these proteins to suit the needs of the virus.

Publications

Key publications: 

  1. B.G. Butt, D.J. Owen, C.M. Jeffries, L. Ivanova, C.H. Hill, J.W. Houghton, M.F. Ahmed, R. Antrobus, D.I. Svergun, J.J. Welch, C.M. Crump, S.C. Graham (2020) Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex. eLife, 9: e53789 [doi: 10.7554/eLife.53789]

  2. T.K. Soh*, C.T.R. Davies*, J. Muenzner*, L.M. Hunter, H.G. Barrow, V. Connor, C.R. Bouton, C. Smith, E. Emmott, R. Antrobus, S.C. Graham^, M.P. Weekes^, C.M. Crump^ (2020) Temporal Proteomic Analysis of Herpes Simplex Virus 1 Infection Reveals Cell-Surface Remodeling via pUL56-Mediated GOPC Degradation. Cell Reports, 33: 108235 [doi: 10.1016/j.celrep.2020.108235]

  3. C. Conceicao*, N. Thakur*, S. Human, J.T. Kelly, L. Logan, D. Bialy, S. Bhat, P. Stevenson-Leggett, A.K. Zagrajek, P. Hollinghurst, M. Varga, C. Tsirigoti, M. Tully, C. Chiu, K. Moffat, A.P. Silesian, J.A. Hammond, H.J. Maier, E. Bickerton, H. Shelton, I. Dietrich, S.C. Graham, D. Bailey (2020) The SARS-CoV-2 Spike protein has a broad tropism for mammalian ACE2 proteins. PLoS Biology 18: e3001016 [doi: 10.1371/journal.pbio.3001016]

  4. C. Gao, M.A. Pallett, T.I. Croll, G.L. Smith, S.C. Graham (2019) Molecular basis of cullin-3 (Cul3) ubiquitin ligase subversion by vaccinia virus protein A55. Journal of Biological Chemistry, 294: 6416–6429 [doi: 10.1074/jbc.RA118.006561]

  5. J.M. Alves, M. Carneiro, J.Y. Cheng, A. Lemos de Matos, M.M. Rahman, L. Loog, P.F. Campos, N. Wales, A. Eriksson, A. Manica, T. Strive, S.C. Graham, S. Afonso, D.J. Bell, L. Belmont, J.P. Day, S.J. Fuller, S. Marchandeau, W.J. Palmer, G. Queney, A.K. Surridge, F.G. Vieira, G. McFadden, R. Nielsen, M.T.P. Gilbert, P.J. Esteves, N. Ferrand, F.M. Jiggins (2019) Parallel adaptation of rabbit populations to myxoma virus. Science, 363: 1319–1326 [doi: 10.1126/science.aau7285]

  6. A. Albecka, D.J. Owen, L. Ivanova, J. Brun, R. Liman, L. Davies, M.F. Ahmed, S. Colaco, M. Hollinshead, S.C. Graham^ and C.M. Crump^ (2017) Dual function of the pUL7-pUL51 tegument protein complex in HSV-1 infection. Journal of Virology, 81: e02196-16 [doi: 10.1128/JVI.02196-16]

  7. J. Muenzner, L.M. Traub, B.T. Kelly^, S.C. Graham^ (2017) Cellular and viral peptides bind multiple sites on the N-terminal domain of clathrin. Traffic, 18: 44–57 [doi: 10.1111/tra.12457]

 (^Joint corresponding authors)

Dr Stephen  Graham
Takes PhD students
Available for consultancy

Affiliations

Classifications: 
Departments and institutes: 
Person keywords: 
virion assembly
immune evasion
molecular biology
host-pathogen interaction
virology
pathogen evolution
structural biology