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Dr Rachael Bashford-Rogers

Dr Rachael Bashford-Rogers

Sir Henry Wellcome Post-doctoral Research Fellow

Department of Medicine, University of Cambridge and Stanford University

Rachael Bashford-Rogers is available for consultancy.


Research Interests

I am interested in determining the immunological changes associated with disease course in a range of diseases using the combination of next-generation sequencing, experimental techniques and computational analyses. By probing genetic diversity of immune receptors in B- and T-cell populations, I aim to answer key questions about disease dynamics in autoimmune disease, infectious disease, immunodeficiency and leukaemia, thus potentially opening avenues for new therapeutic targets, as well as potentially providing a new platform for disease monitoring to improve patient outcomes and quality of life.

 

We have developed highly sensitive molecular frameworks for understanding the dynamics of B- and T-cell populations during infection and malignancy, achieved by high-throughput sequencing, and the development of novel computational methods. Projects range from understanding immune responses in human immunodeficiency virus (HIV), Simian immunodeficiency virus (SIV), Hepatitis B and Hepatitis C in humans and model organisms, to understanding leukaemogenesis in chronic lymphocytic leukaemia (CLL) and acute lymphocytic leukaemia (ALL), and development of diagnostic tools for determining B-cell and T-cell clonality. The work involves multiple national and international collaborations. PhD supervisors: Prof. Paul Kellam and Prof. Allan Bradley, Wellcome Trust Sanger Institute.

Keywords

B cell receptor (BCR) ; B cells ; T cells ; B cell lineage and repertoire development ; T cell receptor (TCR)

Topics

  • haematology
  • autoimmune diseases
  • primary immunodeficiency
  • chronic lymphocytic leukemia
  • lymphoma

Key Publications

Sarah Horton, George Giotopoulos, Haiyang Yun, Shabana Vohra, Olivia Sheppard, Rachael Bashford-Rogers, Mamunur Rashid, Alex Clipson, Wai-In Chan, Daniel Sasca, Loukia Yiangou, Hikari Osaki, Faisal Basheer, Paolo Gallipoli, Natalie Burrows, Aysegul Erdem, Anastasiya Sybirna, Sarah Förster, Wanfeng Zhao, Tonci Sustic, Anna Petrukina Harrison, Elisa Laurenti, Jessica Okosun, Daniel Hodson, Penny Wright, Kenneth Smith, Patrick Maxwell, Jude Fitzgibbon, Ming-Qing Du, David Adams, and Brian Huntly. Early loss of Crebbp confers malignant stem cell properties on lymphoid progenitors. Nature Cell Biology, 2017

Tatsiana Audzevich, Rachel Bashford-Rogers, Neil A. Mabbott, Dan Frampton, Tom C Freeman, Alexandre Potocnik, Paul Kellam, Derek W Gilroy. Novel pre/pro-B cells generates macrophage populations during homeostasis and inflammation. PNAS, 2017

Rachael J. M. Bashford-Rogers, Anne L. Palser, Clare Hodkinson, Joanna Baxter, George A. Follows, George S. Vassiliou, and Paul Kellam. Dynamic variation of CD5 surface expression levels within individual chronic lymphocytic leukaemia clones. Experimental Hematology, 2016.

Bashford-Rogers, R. J., Nicolaou, K. A., Bartram, J., Goulden, N. J., Loizou, L., Koumas, L.,Chi, J., Hubank, M., Kellam, P., Costeas, P. A., Vassiliou, G. S. Eye on the B-ALL: B-cell receptor repertoires reveal persistence of numerous B-lymphoblastic leukemia subclones from diagnosis to relapse. Leukemia, 2016

Kalchschmidt, J. S., Bashford-Rogers, R., Paschos, K., Gillman, A. C., Styles, C. T., Kellam, P., Allday, M. J. "Epstein-Barr virus nuclear protein EBNA3C directly induces expression of AID and somatic mutations in B cells." J Exp Med 2016.

Hoehn KB, Gall A, Bashford-Rogers R, Fidler SJ, Kaye S, Weber JN, McClure MO, Investigators ST, Kellam P, Pybus OG. Dynamics of immunoglobulin sequence diversity in HIV-1 infected individuals. Philosophical transactions of the Royal Society of London Series B, Biological sciences 2015, 370(1676).

R Swann, R Bashford-Rogers, M Robinson, PR Mills, S Barclay, J McLauchlan, P Kellam, A Pate: PTU-110 What impact do hcv infection and subclinical cryoglobulinaemia have on the b cell repertoire? Gut, 2015

McCoy LE, Rutten L, Frampton D, Anderson I, Granger L, Bashford-Rogers R, Dekkers G, Strokappe NM, Seaman MS, Koh W et al: Molecular evolution of broadly neutralizing Llama antibodies to the CD4-binding site of HIV-1. PLoS pathogens 2014, 10(12):e1004552.

Bashford-Rogers RJ, Palser AL, Idris SF, Carter L, Epstein M, Callard RE, Douek DC, Vassiliou GS, Follows GA, Hubank M, Kellam P. Capturing needles in haystacks: a comparison of B-cell receptor sequencing methods. BMC Immunology 2014  5;15(1):29.

Archer EJ, Bashford-Rogers R, Hill B, Douek DC, Kellam P, Koup RA: Evolution of Antigen-specific B-cell Receptor Repertoires in Early SIV Infection. AIDS Res Hum Retroviruses. 2014

Bashford-Rogers RJ, Palser AL, Huntly BJ, Rance R, Vassiliou GS, Follows GA, Kellam P. Network properties derived from deep sequencing of human B-cell receptor repertoires delineate B-cell populations. Genome Research. 2013 Nov;23(11):1874-84. 

Cotten M, Watson SJ, Kellam P, Al-Rabeeah AA, Makhdoom HQ, Assiri A, Al-Tawfiq JA, Alhakeem RF, Madani H, AlRabiah FA, Al Hajjar S, Al-nassir WN, Albarrak A, Flemban H, Balkhy HH, Alsubaie S, Palser AL, Gall A, Bashford-Rogers R, Rambaut A, Zumla AI, Memish ZA. Transmission and evolution of the Middle East respiratory syndrome coronavirus in Saudi Arabia: a descriptive genomic study. Lancet. 2013 

Developed Immune-Network-Generation program that performs novel analyses on B- and T-cell repertoires (publically available for use, 2013).

King ONF, Bashford-Rogers R, Maloney DJ, Jadhav A, Heightman TD, Simeonov A, Clifton IJ, Mcdonough MA, Schofield CJ. Crystal structure of factor inhibiting HIF-1 alpha complexed with inhibitor. PDB entry 3OD4 (2011).