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Dr Manuel D Díaz-Muñoz

Research Interests

Analysis of the implication of ARE binding proteins (AREBPs) in B cell proliferation and plasma cell differentiation through the generation of conditional knock out mice.


signal transduction ; B cells ; protein purification ; microscopy ; confocal microscopy ; differentiation ; interleukin ; cytokines ; memory cells ; B cell receptor (BCR) ; adhesion molecules ; germinal center ; inflammation ; RNA IP ; monocytes ; iCLIP ; RNA and DNA FISH ; immunohistochemistry ; AREBP ; macrophages ; polysome fractionation ; activation ; affinity maturation ; animal models ; traffic ; recombinant protein expression ; immune response ; eicosanoids ; circulation ; statistical analysis ; isotype switching ; inflammatory mediators ; cell culture ; cell development ; fluorescence microscopy ; somatic hypermutation ; FACS ; signalling ; dendritic cells ; migration


  • autoimmunity
  • cardiac immunology
  • cancer
  • cardiac immunity

Key Publications

Díaz-Muñoz MD, Osma-García IC, Iñiguez MA, Fresno M. Cyclooxygenase-2 deficiency in macrophages leads to defective p110γ PI3K signaling and impairs cell adhesion and migration. J Immunol. 2013 Jul 1;191(1):395-406. 

Díaz-Muñoz MD, Osma-García IC, Fresno M, Iñiguez MA. Involvement of PGE2 and the cAMP signalling pathway in the up-regulation of COX-2 and mPGES-1 expression in LPS-activated macrophages. Biochem J. 2012 Apr 15;443(2):451-61.

Iñiguez MA, Punzón C, Cacheiro-Llaguno C, Díaz-Muñoz MD, Duque J, Cuberes R, Alvarez I, Andrés EM, Buxens J, Buschmann H, Vela JM, Fresno M. Cyclooxygenase-independent inhibitory effects on T cell activation of novel 4,5-dihydro-3 trifluoromethyl pyrazole cyclooxygenase-2 inhibitors. Int Immunopharmacol. 2010 Oct;10(10):1295-304.

Díaz-Muñoz MD, Osma-García IC, Cacheiro-Llaguno C, Fresno M, Iñiguez MA. Coordinated up-regulation of cyclooxygenase-2 and microsomal prostaglandin E synthase 1 transcription by nuclear factor kappa B and early growth response-1 in macrophages. Cell Signal. 2010 Oct;22(10):1427-36.

Santos J, González-Sánchez L, Matabuena-Deyzaguirre M, Villa-Morales M, Cozar P, López-Nieva P, Fernández-Navarro P, Fresno M, Díaz-Muñoz MD, Guenet JL, Montagutelli X, Fernández-Piqueras J. A role for stroma-derived annexin A1 as mediator in the control of genetic susceptibility to T-cell lymphoblastic malignancies through prostaglandin E2 secretion. Cancer Res. 2009 Mar 15;69(6):2577-87.

Vila-del Sol V, Díaz-Muñoz MD, Fresno M, Requirement of tumor necrosis factor alpha and nuclear factor-kappaB in the induction by IFN-gamma of inducible nitric oxide synthase in macrophages. J Leukoc Biol. 2007 Jan;81(1):272–83.

Duque J, Díaz-Muñoz MD, Fresno M, Iñiguez MA, Up-regulation of cyclooxygenase-2 by interleukin-1beta in colon carcinoma cells. Cell Signal, 2006 Aug;18(8):1262 9: