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Cambridge Immunology Network

Bacterial immunology


My research focuses on pathogenesis and immunity to bacterial infections,  vaccine development and the interactions between the immune system and antibiotic treatment.

My group has developed new multidisciplinary biological and mathematical approaches that allow in-depth analysis of the complex and multifaceted interactions between bacterial virulence genes, host cell receptors, antimicrobial functions and immune-evasion mechanisms.



Key publications: 

 Key Publications in the last 10 years

  • Fish tank granuloma: an emerging skin disease in Iran mimicking cutaneous leishmaniasis.A. Fata, A. Bojdy,M. Maleki, B. R. Hosseini Farash, K. Ghazvin, P. Tajzadeh, V. Vakili, E. Moghaddas, Pietro Mastroeni, S. Rahmani. PloS One, 2019;
  • The essential role of complement in antibody-mediated resistance to Salmonella. O. Rossi, C. Coward, Y.S. Goh, J. Claassens, C. MacLennan, S. Verbeek, Pietro Mastroeni. Immunology 2019; 156:69-73.doi: 10.1111/imm.13000.
  • Comparative immunogenicity and efficacy of equivalent outer membrane vesicle and glycoconjugate vaccines against nontyphoidal Salmonella. F. Micoli, S. Rondini, R. Alfini, L. Lanzilao, F. Necchi, A. Negrea, O. Rossi, C. Brandt, S. Clare, Pietro Mastroeni, R. Rappuoli, A. Saul, C. MacLennan. Proceedings of the National Academy of Science (PNAS) 2018;
  • A Restricted Role of FcγR in the Regulation of Adaptive Immunity. M. Fransen, W. van Maren, H.Benonisson,H.Sow, C. Breukel, M.Linssen, J. Claassens, C. Brouwers, J. van derKaa, M.Camps,J.Kleinovink, K.Vonk, S. van Heiningen,N. Klar, N.Ho,L.van Beek, V. vanHarmelen,L.Daxinger, K.Nandakumar, R. Holmdahl, C. Coward, Q.Lin, S.Hirose, D.Salvatori, T. vanHall, C.van Kooten,Pietro Mastroeni, F.Ossendorp,S.Verbeek.J. Immunol. 2018;pii: ji1700429. doi: 10.4049/jimmunol.1700429.
  • Expression of Siglec-E alters the phenotype of macrophages but does not affect LPS driven cytokine production or TLR4 endocytosis.  M. Nagala, E. McKenzie, H. Richards, R. Sharma, S. Thomson, Pietro Mastroeni. P. Crocker. Frontiers in Immunol. 2017; doi: 10.3389/fimmu.2017.01926. eCollection 2017.
  • An efficient moments-based inference method for within-host bacterial infection dynamics. D. Price, A. Breuze, Pietro Mastroeni, R. Dybowski, O. Restif. PloS Computational Biology 2017;13(11): e1005841. 
  • Transcriptome and proteome analysis of Salmonella entericaSerovar Typhimurium Infection of wild type C57BL/6 and immunodeficient gp91-/-phoxmice. O. Oshota, M. Fookes, F. Schreiber, R.Chaudhuri, L. Yu, F. Morgan, S. Clare, J. Choudhary, N. Thomson, D. Maskell, Pietro Mastroeni, A. Grant. Plos One 2017; 12(8):e0181365. doi: 10.1371/journal.pone.0181365.
  • Within-host spatiotemporal dynamics of systemic Salmonellainfection during and after antimicrobial treatment. O. Rossi, R. Dybowski, D. Maskell, A. Grant, O. Restif, Pietro MastroeniJ. Antimicrob. Chemother. 2017; 72: 3390-97.
  • Cytotoxicity against tumor cell lines and anti-inflammatory properties of chitinases from Calotropis procera latex. C. Viana, M. V. Ramos, J. Barreto Marinho Filho, L. Lotufo, I. Figueiredo, J. Soares de Oliveira, Pietro Mastroeni, J. Lima-filho, N. Alencar. Naunyn-Schmiedeberg's Archives of Pharmacology 2017; 390:1005-1013.
  • Inferring within-host bottleneck size: a Bayesian approach. R. Dybowski, O. Restif, D. Price, Pietro Mastroeni.Journal of Theoretical Biology 2017; 435:218-228. 
  • Identification and characterisation of a protein involved in Campylobacter jejunicell shape. D. Esson, S. Gupta, D. Bailey, P. Wigley, A. Wedley, A. Mather, G. Méric, Pietro Mastroeni, S. Sheppard, N. Thomson, J. Parkhill, D. Maskell, G. Christie, A.Grant. Microb. Pathogen. 2017; 104:202-211.
  • Effect of in vivoneutralisation of Tumor Necrosis Factor alpha on the efficacy of antibiotic treatment in systemic Salmonella enterica infections.O. Rossi, A. Grant, Pietro MastroeniPathogen. Dis. 2017;doi: 10.1093/femspd/ftx002.
  • Plant lectins ConBr and CFL modulate expression of toll-like receptors besides pro-inflammatory cytokines and reduce the bacterial burden within macrophages infected with Salmonellaentericaserovar Typhimurium.J. Batista, M. Ralph, P. Souza, M. Ramos, Pietro. Mastroeni, J. Lima-Filho. Phytomedicine2017; 25:52-60.
  • Genomic variations leading to alterations in cell morphology of Campylobacterspp. D. Esson, A. Mather, E. Scanlan, S. Gupta, S. de Vries, D. Bailey, S. Harris, T. McKinley, G. Méric, S. Berry, Pietro Mastroeni, S. Sheppard, G. Christie, N. Thomson, J. Parkhill, D. Maskell, A. Grant. Scientific Reports 2016; doi: 10.1038/srep38303.
  • IgG subclasses targeting the flagella of Salmonellaentericaserovar Typhimurium can mediate phagocytosis and bacterial killing. Y. S. Goh, K. Armour, M. Clark, A. Grant, Pietro Mastroeni.Journal of Vaccines and Vaccination 2016; 7: pii:322.
  • Genes required for the fitness of Salmonella entericaserovar Typhimurium during infection of immunodeficient gp91-/-phoxmice. A. Grant, O. Oshota, R. Chaudhuri, M. Mayho, S. Peters, S. Clare, D. Maskell, Pietro MastroeniInfect. Immun. 2016; 84: 989-997.
  • Single passage in mouse organs enhances the survival and spread of Salmonella enterica.R. Dybowski, O. Restif, D. Maskell, Pietro Mastroeni, A. Grant. Royal Society Interface 2015;12:20150702. doi: 10.1098/rsif.2015.0702.
  • TARM1 is a novel leukocyte receptor complex-encoded ITAM receptor that costimulates proinflammatory cytokine secretion by macrophages and neutrophils. V. Radjabova, Pietro Mastroeni, K. Skjodt, P. Zaccone, B. de Bono, J Goodall, E.  Chilvers, J. Juss, D. Jones, J. Trowsdale, A. Barrow. J.  Immunol.2015; 195:3149-3159.
  • Monoclonal antibodies of diverse isotype induced by an O-antigen glycoconjugate vaccine mediate in vitroand in vivokilling of African invasive nontyphoidal SalmonellaY.S. Goh,S. Clare, F. Micoli, A. Saul, Pietro Mastroeni,C. MacLennan. Infect. Immun.2015; 83:3722-3731.
  • Design of glycoconjugate vaccines against invasive African S.Typhimurium. S. Rondini, F. Micoli, L. Lanzilao, M. Gavini, Pietro Mastroeni, C. Brandt, A. Saul, C. MacLennan.  Infect. Immun. 2015; 83:996-1007.
  • The effects of vaccination and immunity on bacterial infection dynamics in vivo. C. Coward, O. Restif, R. Dybowski, A. Grant, D. Maskell, Pietro MastroeniPLoS Pathog. 2014; 10(9):e1004359.
  • Nested sampling for Bayesian model comparison in the context of Salmonelladisease dynamics. R. Dybowski, T. McKinley, Pietro Mastroeni, O. Restif. PLoS one 2013; 8(12):e82317. 
  • Enhanced susceptibility to Citrobacter rodentiuminfection in miR-155- deficient mice.S. Clare, V. John, A. Walker, J. Hill, C. Abreu-Goodger, C. Hale, D. Goulding, T. Lawley, Pietro Mastroeni, G. Frankel, A. Enright, E. Vigorito, G. Dougan. Infect. Immun.2013; 81: 723-732. 
  • Attenuated SalmonellaTyphimurium lacking the pathogenicity island-2 type 3 secretion system grow to high bacterial numbers inside phagocytes in mice. A. Grant,F. Morgan, T. McKinley, G. Foster, D. Maskell, Pietro MastroeniPLoS Pathog. 2012;8: e1003070. 
  • Quantification of the effects of antibodies on the extra- and intracellulardynamics of Salmonella enterica. O. Restif, Y. Goh, M. Palayret, A. Grant, T. McKinley, J. Gog, Pietro MastroeniJ. R. Soc. Interface 2013; 10: 20120866.
  • ASalmonellaTyphimurium-Typhi genomic chimera: a model to study Vi polysaccharide capsule function in vivo. A. Jansen, L. Hall, S. Clare, D. Goulding, K. Holt, A. Grant, Pietro Mastroeni, G. Dougan, R. Kingsley. Plos Pathogens, 2011; 7:e1002131.
  • PD-L1 blockade overrides SalmonellaTyphimurium-mediated diabetes prevention in NOD mice: No role for Tregs. S. Newland, J. Phillips, Pietro Mastroeni, M. Azuma, P. Zaccone, A. Cooke. Eur. J. Immunol.2011; 41:2966-2976.
  • In vivoregulation of the antigen Vi in Salmonellaand induction of immune responses with an in vivoinducible promoter. C. Janis, A. Grant, T. McKinley, F. Morgan, V. John, J. Houghton, R. Kingseley, G. Dougan, Pietro MastroeniInfect. Immun. Spotlight Article 2011; 79: 2481-2488.
  • Human IgG isotypes and activating Fcgamma receptors in the interaction of S.Typhimurium with phagocytic cells. Y.S. Goh, O. Restif, T. McKinley, K. Armour, A. Grant, M. Clark, Pietro MastroeniImmunology2011; 133:74-83.
  • Enhanced virulence of Salmonellaentericaserovar Typhimurium after passage through mice. Pietro Mastroeni, F. Morgan, T. McKinley, G. Foster, E. Shawcroft, S. Clare, D. Maskell, A. Grant.Infect. Immun. 2011; 79:636-643.
  • Anaesthetic impairment of immune function is mediated via GABAreceptors. D. Wheeler, A. Thompson, F. Corletto, J. Reckless, J. Loke, N. Lapaque, A. Grant, Pietro Mastroeni, D. Grainger, C. Padgett, J. O’Brien, N. Miller, J. Trowsdale, S. Lummis, D. Menon, J. Beech. PloS One. 2011; 6: e17152.
  • Copper homeostasis in Salmonellais atypical: contribution to survival inside macrophages. D. Osman, K. Waldron, H. Denton, C. Taylor, A. Grant, Pietro Mastroeni, N. Robinson J. Cavet. J. Biol. Chem. 2010; 285: 25259-25268.
  • TLR and B cell receptor signals to B cells differentially programme primary and memory Th1 responses to Salmonella enterica. T. Barr, S. Brown, Pietro Mastroeni*,D. Gray*.  *Joint senior authorsJ. Immunol. 2010; 185: 2783-2789.
  • Salmonella entericaserovar Typhimurium mutants completely lacking the F0F1ATPase are novel live attenuated vaccine strains. H. Northen, G. Paterson, F. Constantino-Casas, C. Bryant, S. Clare, Pietro Mastroeni, S. Peters, D. Maskell. Vaccine.2010; 28: 940-949.
  • Importance of antibody and complement for oxidative burst and blood cell killing of invasive nontyphoidal Salmonellain Africans. E. Gondwe, M. Molyneux, M. Drayson, S. Graham, Pietro Mastroeni, C.  MacLennan. Proceedings of the National Academy of Science (PNAS) 2010; 107: 3070-3075.
  • Salmonella entericaserovar Typhimurium trxAmutants are protective against virulent challenge but induce less inflammation than the live-attenuated vaccine strain SL3261. S. Peters, G. Paterson, E. Bandularatne, H. Northen, S. Pleasance, C. Willers, J. Wang, A. Foote, F. Constantino-Casas, T. Scase, B. Blacklaws, C. Bryant, Pietro Mastroeni, I. Charles, D. Maskell. Infect. Immun. 2010; 78: 326-336.
  • Toll-like receptor 4 signalling through MyD88 is essential to control Salmonella entericaserovar Typhimurium infection, but not for the initiation of bacterial clearance. S. Talbot, S. Tötemeyer, M. Yamamoto, S. Akira, K. Hughes, D. Gray, T. Barr, Pietro Mastroeni, D. Maskell, C. Bryant. Immunology. 2009; 128: 472-483.
  • LuxS-based quorum sensing does not affect the ability of Salmonella to express the SPI-1 Type 3 secretion system, induce membrane ruffles or invade epithelial cells. C. Perrett, I. Martinez-Argudo, M. Karavolos, H. Spencer, D. Bulmer, Pietro Mastroeni,K. Winzer,  E. McGhie, V. Koronakis, P. Williams, A. Khan, M. Jepson. J. Bacteriol. 2009; 191: 7253-7259.
  • Bacterial growth rate and host factors as determinants of intracellular bacterial distributions in systemic Salmonella entericainfections. A. Grant, G. Foster, T. McKinley, S. Brown, S. Clare, M. Sheppard, D. Maskell, Pietro MastroeniInfect. Immun. 2009; 77: 5608-5611.
  • Multiple redundant stress resistance mechanisms are induced in Salmonella entericaserovar Typhimurium in response to alteration of the intracellular environment via TLR4 signalling. J. Wright, S. Tötemeyer, I. Hautefort, C. Appia-Ayme, M. Alston, V. Danino, G. Paterson, Pietro Mastroeni, N. Ménager, M. Rolfe, A.Thompson, S. Ugrinovic, L. Sait, T. Humprey, H. Northen, S. Peters, D. Maskell, J. Hinton, C. Bryant. Microbiology2009; 155: 2919-2929.
  • Antibiotic treatment of Clostridium difficilecarrier mice triggers a supershedder state, transmission and disease in immunocompromised hosts. T. Lawley, S. Clare, A. Walker, D. Goulding, R. Stabler, N. Croucher, Pietro Mastroeni, P. Scott, C. Raisen, L. Mottram, N. Fairweather, B. Wren, J. Parkhill, G. Dougan. Infect. Immun. 2009;77: 3661-3669.
  • B-cell intrinsic MyD88 signals drive IFN-γ production from T cells and control switching to IgG2c. T. Barr, S. Brown, Pietro Mastroeni, D. Gray. J. Immunol. 2009; 183: 1005-1012.


Dr Pietro  Mastroeni
Takes PhD students
Available for consultancy


Departments and institutes: 
Person keywords: 
B cells
confocal microscopy
molecular biology
B cell receptor (BCR)
enzyme assays
Fc receptors
T cells
human studies
antibody responses
animal models
helper T cells
cell culture
fluorescence microscopy